We use the unique sgRNA screening technology to select the most efficient sgRNA from 4-6 sgRNA for each gene to construct sgRNA lentivirus library. Now we provide dozens of sgRNA lentivirus libraries including apparent regulatory factors, kinase libraries, tumor target gene libraries, ubiquitin linked enzyme gene libraries and various signal pathways. Each gene plasmid in all libraries is independently screened. The construction ensures 100% coverage and knockout efficiency of the library genes, and greatly improves the success rate of screening.
Our existing library can provide two library forms: Mixed Library and single gene independent library according to customer needs. At the same time, we use our existing library and mature high-throughput screening platform to provide customized high-throughput genetic function screening services for customers.
If the product you need is not in our product list, you only need to provide gene name, we will provide you with a quick and efficient one-stop gene editing cell service through our mature technology platform.
See the following table for the directory of existing libraries:
| Product name（English） | Product name（Chinese） | Gene number |
|---|---|---|
| Human m6A modification genes Library | m6A修饰相关基因文库 | 22 |
| Human Epigenetic regulators Library | 表观调控因子文库 | 423 |
| Human Kinases Library | 激酶文库 | 403 |
| Human Phosphatase Library | 磷酸酶文库 | 198 |
| Human Ubiquitin Ligase Library | 泛素连接酶文库 | 603 |
| Human Cancer target Library | 肿瘤靶点基因文库 | 96 |
| Human Cytokine and Chemokine Library | 细胞因子和趋化因子文库 | 134 |
| Human GPCR Library | GRCR文库 | 280 |
| Human Diabetes related genes Library | 糖尿病相关基因文库 | 130 |
| Human Histone Modification Library | 组蛋白修饰基因文库 | 332 |
| Human Oncogenes Library | 癌基因文库 | 326 |
| Human Tumor metastasis genes Library | 肿瘤侵袭相关基因文库 | 60 |
| Human Tumor suppressor genes Library | 肿瘤抑制基因文库 | 718 |
| Human ES Cell Differentiation Library | 干细胞分化基因文库 | 334 |
| Human Wnt signaling pathways Library | Wnt信号通路文库 | 491 |
| Human cAMP&Ca Signaling Pathway Library | cAMP&Ca信号通路文库 | 91 |
At the same time, we provide the customized service of gRNA library, and specially design the library of specific signal pathway according to the needs of customers, such as disease-related pathway: tumor suppressor gene, proto oncogene, diabetes gene, cardiovascular disease gene, Parkinson's disease gene, etc.; signal transduction pathway: Ras signal pathway, Wnt signal pathway, JAK-STAT signal pathway, NF-kB signal pathway, TNF signal pathway. P13k-akt signaling pathway; cell function pathway: autophagy pathway, cell cycle pathway, apoptosis pathway, etc. In addition, according to the specific signal path customization needs of customers, we can design gRNA for customization. Customers only need to provide relevant gene names.
We provide customized high-throughput genetic function screening services, including 1) cell proliferation related gene screening (in vitro screening) 2) cell migration / invasion related gene screening (in vitro screening) 3) cell clone formation related gene screening (in vitro screening) 4) tumor resistance related gene screening (in vitro screening) 5) cell signal pathway related gene screening (in vitro screening) 6) swelling Screening of tumor metastasis related genes (in vivo screening) 7) screening of other functional genes, such as autophagy, cell differentiation, etc.
High throughput gene function screening is the source of original innovation in the biomedical field. Recently, many gene function screening works have been published by using the whole genome sgRNA library constructed by Zhang Feng lab. however, the market library cannot guarantee the actual coverage and uniformity of genes due to multiple generations, and our library can guarantee 10% due to screening one sgRNA / gene and independent construction. 0% gene coverage and homogeneity can greatly improve the success rate of screening.
| Our whole genome sgRNA Library | Full gene library in the current market(FengZhang, MIT） |
|---|---|
| 1.Single gene independently constructed | 1.All genes mixed |
| 2.The sgRNA of each gene was screened and there was only one sgRNA. | 2.Each gene has a huge capacity of 6-10 sgRNA designed by computer. |
| 3.The knockout effect of each gene is verified to ensure the coverage of the whole gene. | 3.The knockout effect of gene has not been verified, and it can not be guaranteed to cover the whole genome through multiple generations. |
| 4.The size of the library can be customized according to the needs, such as the construction of kinase library and oncogene library. | 4.It's not customizable, it's a whole genome. |
| 5.Both positive and negative screening can be carried out. | 5.Only positive screening can be carried out |
| 6.Hybrid Library and single play independent Library | 6.Mixed library only |
Case 1: the role of CRISPR genomic screening signaling pathway research
Case 2: the role of CRISPR genome screening in the discovery of new tumor immune regulatory factors
Related downloads: High throughput genetic screening of cas9 gene knockout Library
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