Lentivirus system can efficiently transfer genes into living model animals and almost all mammalian cells, including undifferentiated, inactive or stopped growing cells, including neuron cells, primary cells, stem cells. The efficiency of lentivirus transduction is close to 100%. It is an ideal expression vector system.

The expression vector types of lentivirus include n or C-terminal with fusion label or without label. The vector contains sequence elements that can be efficiently packaged, transfected and stably integrated into the genome of the target cell, so that our expression clone can be efficiently expressed in the target cell.

Four sgRNAs were designed for each gene to deliver one sgRNA with the highest efficiency.