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Crispr/Cas9 Rapid establishment of specific gene knockout cell lines

2018-08-26

基因治疗

Crispr/Cas9 Rapid establishment of specific gene knockout cell lines

Crispr/Cas9 system is currently the most efficient genome editing system, which has been applied to a variety of organisms, including human cells, zebrafish, mice, rats, Caenorhabditis elegans, plants and bacteria.

Shanghai Chengzhi Biotechnology Inc., developed the CRISPR / cas9 lentivirus infection system to rapidly construct specific gene knock-out cell lines. Through the verification and screening of gRNA, the stable cell lines with effective gene knock-out were constructed, and the customer's verified gene knock-out cell lines were quickly and efficiently provided.

Product advantage

1. Simply   Provide gene ID and one-stop service to obtain the knockout polyclonal cell line for functional research

2. Fast  20-30 working days to obtain the required knockout polyclonal cell line

3. Effective  Ensure gene knockout effect by screening

4.Contrast   At the same time, we provide the customer with the control cell line without gene knockout.

Service process

1. The user provides the gene ID and cell line. If the cell line is commonly used, the user does not need to provide it, but if it is a special cell line, the user needs to provide the cell line and culture conditions.

2. Sign technical service agreement and carry out technical services.

3. According to the difference of cell growth conditions, provide two tubes of gene knock-out cell lines and non knock out control cell lines required by customers within 20-30 working days, each tube of cells shall not be less than 1 million.

Service instance

We constructed a gene knockout cell line based on the G2 gRNA of Caspase-3 gene. Western blot showed that caspase-3 protein knockout was complete, and the knockout of Caspase-3 could effectively resist apoptosis.

基因治疗

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